Journal
SCIENCE
Volume 325, Issue 5941, Pages 764-767Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1175653
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Funding
- NIH [R37AI033493, R01AI055977, R01AI044239, T32GM08061]
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Pathogens can use DNA recombination to promote antigenic variation (Av) of surface structures to avoid immune detection. We identified a cis-acting DNA sequence near the antigenically variable pilin locus of the human pathogen, Neisseria gonorrhoeae. This 16-base pair guanine (G)-rich sequence was required for pilin Av and formed a guanine quartet (G4) structure in vitro. Individual mutations that disrupted the structure also blocked pilin Av and prevented nicks required for recombination from occurring within the G4 region. A compound that binds and stabilizes G4 structures also inhibited pilin Av and prevented nicks from occurring on the G-rich strand. This site constitutes a recombination initiation sequence/structure that directs gene conversion to a specific chromosomal locus.
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