Journal
SCIENCE
Volume 325, Issue 5945, Pages 1240-1243Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1177321
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Funding
- Wellcome Trust Senior Fellowship [064414]
- UK Biotechnology and Biological Sciences Research Council
- Medical Research Council (UK)
- European Union
- European Molecular Biology Organization
- Human Frontier Science Program Organization
- Louis-Jeantet Foundation
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Posttranslational modifications play key roles in regulating chromatin plasticity. Although various chromatin-remodeling enzymes have been described that respond to specific histone modifications, little is known about the role of poly[adenosine 5'-diphosphate (ADP)-ribose] in chromatin remodeling. Here, we identify a chromatin-remodeling enzyme, ALC1 (Amplified in Liver Cancer 1, also known as CHD1L), that interacts with poly(ADP-ribose) and catalyzes PARP1-stimulated nucleosome sliding. Our results define ALC1 as a DNA damage-response protein whose role in this process is sustained by its association with known DNA repair factors and its rapid poly(ADP-ribose)-dependent recruitment to DNA damage sites. Furthermore, we show that depletion or overexpression of ALC1 results in sensitivity to DNA-damaging agents. Collectively, these results provide new insights into the mechanisms by which poly(ADP-ribose) regulates DNA repair.
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