Journal
SCIENCE
Volume 326, Issue 5950, Pages 257-263Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1179050
Keywords
-
Categories
Funding
- American Physicians Fellowship for Medicine in Israel
- NIH [R21 AI71060]
- Burroughs Wellcome Fund
- Sloan Foundation
Ask authors/readers for more resources
Models of mammalian regulatory networks controlling gene expression have been inferred from genomic data but have largely not been validated. We present an unbiased strategy to systematically perturb candidate regulators and monitor cellular transcriptional responses. We applied this approach to derive regulatory networks that control the transcriptional response of mouse primary dendritic cells to pathogens. Our approach revealed the regulatory functions of 125 transcription factors, chromatin modifiers, and RNA binding proteins, which enabled the construction of a network model consisting of 24 core regulators and 76 fine-tuners that help to explain how pathogen-sensing pathways achieve specificity. This study establishes a broadly applicable, comprehensive, and unbiased approach to reveal the wiring and functions of a regulatory network controlling a major transcriptional response in primary mammalian cells.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available