Journal
SCIENCE
Volume 324, Issue 5931, Pages 1203-1206Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1168729
Keywords
-
Categories
Funding
- NIH [GM049985, GM036559, GM041455]
- Leukemia and Lymphoma Society
- NIH Pathway to Independence Award [K99 GM085136]
- NIH Roadmap for Medical Research Grant [U54 GM072970]
Ask authors/readers for more resources
Transcribing RNA polymerases oscillate between three stable states, two of which, pre- and posttranslocated, were previously subjected to x-ray crystal structure determination. We report here the crystal structure of RNA polymerase II in the third state, the reverse translocated, or backtracked state. The defining feature of the backtracked structure is a binding site for the first backtracked nucleotide. This binding site is occupied in case of nucleotide misincorporation in the RNA or damage to the DNA, and is termed the P site because it supports proofreading. The predominant mechanism of proofreading is the excision of a dinucleotide in the presence of the elongation factor SII (TFIIS). Structure determination of a cocrystal with TFIIS reveals a rearrangement whereby cleavage of the RNA may take place.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available