Journal
SCIENCE
Volume 324, Issue 5929, Pages 927-928Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1169237
Keywords
-
Categories
Funding
- NIH [GM41376]
- Howard Hughes Medical Institute
- Pew Scholars Award
Ask authors/readers for more resources
During transcription initiation in vitro, prokaryotic and eukaryotic RNA polymerase ( RNAP) can engage in abortive initiation-the synthesis and release of short (2 to 15 nucleotides) RNA transcripts-before productive initiation. It has not been known whether abortive initiation occurs in vivo. Using hybridization with locked nucleic acid probes, we directly detected abortive transcripts in bacteria. In addition, we show that in vivo abortive initiation shows characteristics of in vitro abortive initiation: Abortive initiation increases upon stabilizing interactions between RNAP and either promoter DNA or sigma factor, and also upon deleting elongation factor GreA. Abortive transcripts may have functional roles in regulating gene expression in vivo.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available