Journal
RSC ADVANCES
Volume 5, Issue 120, Pages 99439-99447Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5ra14982g
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Funding
- Special Fund for Forest Scientific Research in the Public Welfare [201504501]
- Fundamental Research Funds for the Central Universities [2572014AB13]
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A novel magnetically separable laccase immobilized system was constructed by immobilizing Aspergillus laccase on the core-shell structure of amino-functionalised magnetic silica nanoparticles (AF-MSNPs) via a chemical crosslinking method. A large immobilization capacity (613.5 +/- 10.5 mg g(-1)), activity recovery of 53.4 +/- 3.1% and broader pH and temperature profiles than free laccase have been exhibited by the immobilized laccase. Thermal stability, storage stability and operational stability were increased to a great extent. The application of the immobilized system in catalyzing the guaiacol as a phenolic lignin model compound was investigated. The high catalytic efficiency for guaiacol indicated that utilizing immobilized laccase to catalyse lignin is a very promising technology for further applications in the wood industry.
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