Journal
SCIENCE
Volume 320, Issue 5879, Pages 1077-1081Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1157396
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Funding
- Howard Hughes Medical Institute Funding Source: Medline
- NHGRI NIH HHS [R01 HG003367-03, HG003367, R01 HG003367] Funding Source: Medline
- NIAID NIH HHS [P30 AI042845-119008, P30 AI042845, R01 AI043208, R01 AI043208-08] Funding Source: Medline
- NIA NIH HHS [F30AG030283, F30 AG030283-04, F30 AG030283-03, F30 AG030283-02] Funding Source: Medline
- NIGMS NIH HHS [R01 GM080625, R01 GM080625-02, R37 GM062862-11, R01 GM065236-08, GM080625, R37 GM062862, R01 GM065236, GM62862, R01 GM062862-09, R01 GM065236-07, GM65236, R01 GM062862, R01 GM080625-03, R01 GM062862-08] Funding Source: Medline
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Small interfering RNAs ( siRNAs) direct RNA interference ( RNAi) in eukaryotes. In flies, somatic cells produce siRNAs from exogenous double- stranded RNA ( dsRNA) as a defense against viral infection. We identified endogenous siRNAs ( endo- siRNAs), 21 nucleotides in length, that correspond to transposons and heterochromatic sequences in the somatic cells of Drosophila melanogaster. We also detected endo- siRNAs complementary to messenger RNAs ( mRNAs); these siRNAs disproportionately mapped to the complementary regions of overlapping mRNAs predicted to form double- stranded RNA in vivo. Normal accumulation of somatic endo- siRNAs requires the siRNA- generating ribonuclease Dicer- 2 and the RNAi effector protein Argonaute2 ( Ago2). We propose that endo- siRNAs generated by the fly RNAi pathway silence selfish genetic elements in the soma, much as Piwi- interacting RNAs do in the germ line.
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