Journal
SCIENCE
Volume 321, Issue 5885, Pages 133-136Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1159419
Keywords
-
Categories
Funding
- NIAMS NIH HHS [P01 AR051174-050003, AR051174, P01 AR051174] Funding Source: Medline
- NIGMS NIH HHS [GM057247, R01 GM057247-10, R01 GM057247] Funding Source: Medline
Ask authors/readers for more resources
The ability to sense molecular tension is crucial for a wide array of cellular processes, including the detection of auditory stimuli, control of cell shape, and internalization and transport of membranes. We show that myosin I, a motor protein that has been implicated in powering key steps in these processes, dramatically alters its motile properties in response to tension. We measured the displacement generated by single myosin I molecules, and we determined the actin-attachment kinetics with varying tensions using an optical trap. The rate of myosin I detachment from actin decreases > 75- fold under tension of 2 piconewtons or less, resulting in myosin I transitioning from a low (< 0.2) to a high (> 0.9) duty- ratio motor. This impressive tension sensitivity supports a role for myosin I as a molecular force sensor.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available