Journal
SCIENCE
Volume 322, Issue 5906, Pages 1353-1356Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1165401
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Funding
- Institut de Recherches International Servier
- Association pour la Recherche sur le Cancer
- French Ministry of Research
- Association Francaise contre les Myopathies
- CNRS
- Agence Nationale de la Recherche
- Human Frontier Science Program Organization
- ARC
- French Ministry of Research [ACI-BCMS 338]
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Microtubules display dynamic instability, with alternating phases of growth and shrinkage separated by catastrophe and rescue events. The guanosine triphosphate (GTP) cap at the growing end of microtubules, whose presence is essential to prevent microtubule catastrophes in vitro, has been difficult to observe in vivo. We selected a recombinant antibody that specifically recognizes GTP- bound tubulin in microtubules and found that GTP- tubulin was indeed present at the plus end of growing microtubules. Unexpectedly, GTP- tubulin remnants were also present in older parts of microtubules, which suggests that GTP hydrolysis is sometimes incomplete during polymerization. Observations in living cells suggested that these GTP remnants may be responsible for the rescue events in which microtubules recover from catastrophe.
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