Journal
RSC ADVANCES
Volume 5, Issue 122, Pages 100960-100967Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5ra18918g
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Funding
- National Natural Science Foundation of China [21405130]
- Excellent Talents of Xuzhou Medical College [D2014007]
- State Key Laboratory of Analytical Chemistry for Life Science [SKLACLS1405]
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In this study, a sensitive protocol for the high-performance liquid chromatography (HPLC) detection of adenosine triphosphate (ATP) based on mesoporous silica nanoparticles (MSN) functionalized with an aptamer as a cap has been designed. Aminopyrine (AP) is sealed in the inner pores of MSN with single-stranded DNA. In the presence of ATP, an ATP aptamer combines with ATP and escapes from a pore and thus opens the DNA biogate to release AP. The released signal tags can be easily read out by HPLC. The designed protocol provides sensitive HPLC detection of ATP down to 0.41 nM with a linear range of four orders of magnitude (from 1.0 nM to 10 mu M) and has high selectivity toward its target ATP, which can be attributed to the large loading capacity and highly ordered pore structure of mesoporous silica nanoparticles, as well as the selective binding of the aptamer with ATP. This proof of concept might promote the application of ATP-responsive devices and also provide ideas for designing various target-responsive systems using other aptamers as caps.
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