Journal
SCIENCE
Volume 321, Issue 5892, Pages 1081-1084Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1158013
Keywords
-
Categories
Funding
- Intramural NIH HHS [Z01 AI000403-24] Funding Source: Medline
Ask authors/readers for more resources
In T cells, the stochasticity of protein expression could contribute to the useful diversification of biological functions within a clonal population or interfere with accurate antigen discrimination. Combining computer modeling and single- cell measurements, we examined how endogenous variation in the expression levels of signaling proteins might affect antigen responsiveness during T cell activation. We found that the CD8 co- receptor fine- tunes activation thresholds, whereas the soluble hematopoietic phosphatase 1 ( SHP- 1) digitally regulates cell responsiveness. Stochastic variation in the expression of these proteins generates substantial diversity of activation within a clonal population of T cells, but co- regulation of CD8 and SHP- 1 levels ultimately limits this very diversity. These findings reveal how eukaryotic cells can draw on regulated variation in gene expression to achieve phenotypic variability in a controlled manner.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available