Association of IRF5 gene polymorphisms with rheumatoid arthritis in a Tunisian population

Objective: A strong genetic association of rheumatoid arthritis (RA) with the interferon regulatory factor 5 (IRF5) gene has been described previously in a Swedish population, although this result was not confirmed in a French population. We undertook an association study between IRF5 and the RA phenotype, as well as a study with serological markers of RA, in a Tunisian population. Methods: A single-nucleotide polymorphism (SNP; rs2004640) was genotyped using a Taqman 5' allelic discrimination assay on an ABI 7500 real-time polymerase chain reaction (PCR) instrument in 140 RA patients and 185 controls. Rheumatoid factor (RF) and anti-citrullinated protein/peptide antibodies (ACPA) were determined by enzyme-linked immunosorbent assay (ELISA). Association was assessed based on the chi(2) test and odds ratios (ORs) with 95% confidence intervals (CIs). Results: The frequency of the TT genotype of the IRF5 SNP rs2004640 differed significantly between patients and controls (p=0.01). This difference was greater when a subgroup of patients with another 'autoimmune' disorder was considered (p=0.007). A weak but significant association was also found in a subgroup of patients who were positive for ACPA (p=0.04) or erosion (p=0.01). Conclusions: Our results indicate that the TT genotype of the IRF5 (rs2004640) dimorphism is associated with RA in a Tunisian population.