Journal
SCANDINAVIAN JOURNAL OF MEDICINE & SCIENCE IN SPORTS
Volume 21, Issue 2, Pages 215-223Publisher
WILEY
DOI: 10.1111/j.1600-0838.2009.01044.x
Keywords
GDF-8; growth factor; cytokine
Categories
Funding
- University of Copenhagen
- Copenhagen Graduate School of Health Sciences, University of Copenhagen
- Danish National Research Foundation [504-14]
- Capital Region of Denmark (Region H)
Ask authors/readers for more resources
Myostatin is a potent negative regulator of skeletal muscle mass, but its role in human skeletal muscle hypertrophy and atrophy is sparsely described. Muscle biopsies were obtained from young male subjects before and after 30 and 90 days of resistance training as well as after 3, 10, 30, 60 and 90 days of subsequent detraining. Myostatin mRNA increased significantly with detraining. We observed a 28 kDa myostatin immunoreactive protein, which, however, was also present in myostatin knock out mice skeletal muscle. As a novel finding we consistently detected a 10 kDa band, which may represent a mature myostatin monomer under reducing conditions or a novel, unknown myostatin form. Further, we observed a significant increase in this 10 kDa band after 3 days of detraining preceding the rapid type II fiber atrophy, in which almost half of the acquired fiber area was lost after only 10 days of detraining. Accordingly, an increase in the level of the 10 kDa protein is associated with rapid type II fiber atrophy, suggesting myostatin-mediated specific type II fiber atrophy, which in combination with our mRNA data support a role for myostatin in the negative regulation of adult human skeletal muscle mass.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available