Journal
RSC ADVANCES
Volume 5, Issue 58, Pages 46686-46693Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5ra08843g
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Funding
- Japan Society for the Promotion of Science (JSPS) [25288101]
- Cooperative Research Program, Network Joint Research Center for Materials and Devices [2013B25]
- Grants-in-Aid for Scientific Research [25288101] Funding Source: KAKEN
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Poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) possesses protein antifouling properties. Diblock copolymers (PMPC120-b-P(TSM/CEAx)(y)) composed of a PMPC block and random copolymer block with 3-(tris(trimethylsiloxy)silyl)propyl methacrylate (TSM) and 2-cinnamoylethyl acrylate (CEA) were prepared via reversible addition-fragmentation chain transfer (RAFT) radical polymerization. A thin film of PMPC120-P(TSM/CEAx)(y) formed on the surface of the poly(dimethylsiloxane) (PDMS) substrate due to physical adsorption of the TSM units to poly(dimethylsiloxane) (PDMS) and photo-crosslinking of the CEA units. A lattice pattern of PMPC120-P(TSM/CEAx)(y) on the PDMS surface was prepared using UV irradiation through a photomask. PMPC120-P(TSM/CEAx)(y)-coated PDMS demonstrated protein antifouling activity. Cell patterning could be achieved by culturing on the PMPC-patterned PDMS substrate.
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