Journal
RSC ADVANCES
Volume 5, Issue 76, Pages 61906-61912Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5ra12527h
Keywords
-
Categories
Funding
- European Union [258759]
Ask authors/readers for more resources
Human C-reactive protein (CRP) has been reported as an inflammatory biomarker with the highest reference for use in clinical practice. However, the existing analytical techniques are lacking automation and simplicity, as desired for a prospective immunoassay format for point-of-care (PoC) analysis. We have developed an automated magnetic chemiluminescent immunoassay (MCIA) on a mobile analyser for rapid PoC determination of CRP. The MCIA is fully automated after the initial loading of sample and immunoreagents at the inlet ports. The automated protocol involves the transportation of magnetic capture microparticles between adjacent reaction compartments using a set of stationary magnets, a microfluidic polymer disposable and a specific centrifugal protocol. The developed MCIA has a sample-to-answer time of 25 min and hands-on time of approximately 5 min. It detects the entire pathophysiological range of CRP, as desired for clinically-relevant high sensitivity CRP immunoassay format, i.e. 3-81 ng mL(-1) in diluted human serum with a limit of detection (LOD) and limit of quantification (LOQ) of 1.5 ng mL(-1) and 1.8 ng mL(-1), respectively.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available