Journal
RUSSIAN JOURNAL OF PLANT PHYSIOLOGY
Volume 61, Issue 1, Pages 124-130Publisher
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1134/S102144371401018X
Keywords
Chlorella pyrenoidosa; microalgae; autoselection; productivity; fuel oil producers; biodiesel
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Funding
- State Order within a Framework of the Budget Program 120 on Grant Financing of Scientific Research of Republic of Kazakhstan [0112RK00331]
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Algalogically pure cultures were isolated from different aquatic ecosystems and identified as representatives of green algae, euglena algae, and cyanobacteria. Isolated cultures and collection strains of microalgae were screened for productivity. Induced mutagenesis (exposure to UV radiation, 254 nm, 40 Erg/mm(2)) and selection yielded a mutant strain C-2m2 of Chlorella pyrenoidosa notable for a high activity of lipid biosynthesis and accumulation. Optimization of its culture conditions was conducted. It was found that the active lipid accumulation by the cells of C. pyrenoidosa strain C-2m2 occurred when nitrogen concentration in the medium was tenfold reduced (to the level of 0.004 g/L) at an illuminance of 4 klx.
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