Journal
RUSSIAN JOURNAL OF PLANT PHYSIOLOGY
Volume 55, Issue 2, Pages 256-258Publisher
PLEIADES PUBLISHING INC
DOI: 10.1134/S1021443708020143
Keywords
triticum aestivum; osmotin; osmotolerance; salt stress; salinity tolerance; transformation
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The genetically engineered osmotin gene located in a binary vector was transferred by shotgun to an Iranian wheat cultivar (Triticurn aestivum, cv. Marvdasht):Screening of transgenic plants was carried out by using both molecular and phenotypic approaches. Transgenic lines were studied for their ability to tolerate salinity stress at various concentrations of NaCl. Transgenic line of cv. Marvdasht exhibited a high ability to produce roots at the concentration of 250 mM NaCl. In this research, a resistant Iranian cv. Zarrin and nontransgenic lines of cv. Marvdasht were also compared for their root length under salinity condition. Results showed that trans-enic Marvdasht lines are significantly more tolerant than nontransgenic ones and very similar to naturally resistant Zarrin in this aspect.
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