A novel colorimetric and near-infrared fluorescent probe for hydrogen peroxide imaging in vitro and in vivo

A novel NIR fluorescent probe (DCM-B2) based on dicyanomethylene-4H-pyran was synthesized for the detection of H2O2. This colorimetric fluorescent probe displays a fluorescence turn-on response following the conversion of the aryl boronate unit to phenol in the presence of H2O2. It could offer good performance in terms of sensitivity, selectivity, and low cytotoxicity. Furthermore, bioimaging investigations indicated that this probe was cell permeable and suitable for monitoring H2O2 in vitro and in vivo.