Linkage of a RAPD marker with powdery mildew resistance er-1 gene in Pisum sativum L.

The aim of this study was to investigate the inheritance of powdery mildew disease and to tag it with a DNA marker to utilize for the marker-assisted selection (MAS) breeding program. The powdery mildew resistant genotype Fallon (er) and susceptible genotype 11760-3 (ER) were selected from 177 genotypes by heavy infestation of germplasm with Erysiphe pisi through artificial inoculation The F-1 plants of the cross Fallon/11760-3 indicated the dominance of the susceptible allele, while F-2 plants segregated in 3: 1 ratio (susceptible: resistant) that fit for goodness of fitness by chi(2) (P > 0.07), indicating monogenic recessive inheritance for powdery mildew resistance in Pisum sativum. A novel RAPD marker OPB18 (5'-CCACAGCAGT-3') was linked to the er-1 gene with 83% probability with a LOD score of 4.13, and was located at a distance of 11.2 cM from the er-1 gene.