Journal
RSC ADVANCES
Volume 5, Issue 76, Pages 61639-61649Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5ra11713e
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Funding
- Russian Scientific Foundation [14-13-01167, 14-15-00186]
- [14.Z50.31.0004]
- Russian Science Foundation [14-15-00186, 14-13-01167] Funding Source: Russian Science Foundation
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Fluorescent Au nanoclusters (NCs) have been the subject of intense study owing to their increasing applications in imaging, sensing, and nanomedicine. Herein, highly fluorescent bovine serum albumin (BSA)-directed Au-BSA NCs are synthesized as a platform for further conjugation with human antistaphylococcal immunoglobulin (antiSAIgG) and the photodynamic (PD) synthesizer Photosens (TM) (PS) to fabricate Au-BSA-antiSAIgG-PS complexes. The complexes possess three theranostic modalities: biospecific detection of Staphylococcus aureus bacteria, intense red fluorescence due to Au-BSA NCs (quantum yield, similar to 14%), and synergistic PD inactivation due to the PS dye. Owing to biospecific targeting and intense red fluorescence, the Au-BSA-antiSAIgG probe can detect pathogenic bacteria in bacterial mixtures through fluorescence microscopy or even through naked eye inspection of sediments under UV illumination. The developed complexes can be used at a physiological pH of similar to 7, in contrast to nonspecific electrostatic binding of Au-human serum albumin NCs to S. aureus at pH < 5-6. PD treatment of methicillin-sensitive and methicillin-resistant S. aureus with Au-BSA-antiSAIgG-PS complexes and 660 nm light irradiation significantly inactivates both types of bacteria. By using the developed strategy, Au-BSA-PS complexes can be further modified with other targeting and chemotherapeutic molecules, thus ensuring new theranostic applications in current nanomedicine.
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