Journal
RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY
Volume 38, Issue 5, Pages 488-499Publisher
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1134/S1068162012050068
Keywords
modified oligoribonucleotides; cross-linking; RNA-protein interactions; bacterial ribonuclease P
Funding
- RFBR-DFG program International Research Group with the Participation of Young Scientists [11-04-91337 DFG_a, IRTG GRK 1384]
- RFBR [10-04-01578_a]
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1,2-Diol-oligoribonucleotides were prepared using fully protected 2'-O-[2-(2,3-dihydroxypropyl)amino-2-oxoethyl]uridine 3'-phosphoramidite. Incorporation of the modified uridine residue into oligonucleotide chains was not shown to significantly affect the thermal stability of RNA-RNA and RNA-DNA duplexes. Periodate oxidation of the 1,2-diol group resulted in reactive 2'-aldehyde oligoribonucleotides. These oligonucleotides were studied for their application in the affinity modification of RNA recognizing proteins with an example of bacterial ribonuclease P.
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