Journal
RNA
Volume 17, Issue 12, Pages 2063-2073Publisher
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1261/rna.02890211
Keywords
ribosome; footprinting; codon usage; translation; wobble
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Funding
- NIGMS [RO1GM37706]
- NIAID [U54065359]
- Stanford Genome Training Program [T32-HD00044]
- Stanford Graduate Fellowship Program
- National Science Foundation
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In the universal genetic code, most amino acids can be encoded by multiple trinucleotide codons, and the choice among available codons can influence position-specific translation elongation rates. By using sequence-based ribosome profiling, we obtained transcriptome-wide profiles of in vivo ribosome occupancy as a function of codon identity in Caenorhabditis elegans and human cells. Particularly striking in these profiles was a universal trend of higher ribosome occupancy for codons translated via G:U wobble base-pairing compared with synonymous codons that pair with the same tRNA family using G:C base-pairing. These data support a model in which ribosomal translocation is slowed at wobble codon positions.
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