Journal
RNA
Volume 16, Issue 4, Pages 817-827Publisher
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1261/rna.1712910
Keywords
BMV; LSm1-7; RNA virus; Sm proteins; translation; replication
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Funding
- Spanish Ministerio de Educacion y Ciencia [BFU2007-66933/BMC]
- German Research Foundation [DFG-FOR855]
- Fundacao para a Ciencia e Tecnologia [SARH/BD/9630/2002, SFRH/BD/37047/2007]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/37047/2007] Funding Source: FCT
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LSm1-7 complexes promote cellular mRNA degradation, in addition to translation and replication of positive-strand RNA viruses such as the Brome mosaic virus (BMV). Yet, how LSm1-7 complexes act on their targets remains elusive. Here, we report that reconstituted recombinant LSm1-7 complexes directly bind to two distinct RNA-target sequences in the BMV genome, a tRNA-like structure at the 3'-untranslated region and two internal A-rich single-stranded regions. Importantly, in vivo analysis shows that these sequences regulate the translation and replication of the BMV genome. Furthermore, both RNA-target sequences resemble those found for Hfq, the LSm counterpart in bacteria, suggesting conservation through evolution. Our results provide the first evidence that LSm1-7 complexes interact directly with viral RNA genomes and open new perspectives in the understanding of LSm1-7 functions.
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