Journal
RNA
Volume 15, Issue 11, Pages 2057-2062Publisher
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1261/rna.1670909
Keywords
exoribonuclease; purification; detection; inhibitor; activator; screening
Categories
Funding
- Centre National de la Recherche Scientifique [UPR9073]
- Universite Paris VII-Denis Diderot
- Association pour la Recherche sur le Cancer [ARC A07/2/4831]
- ANR blanc (REGULncRNA)
- Ministere pour la Recherche et la Technologie (MNRT)
Ask authors/readers for more resources
The identification of RNases or RNase effectors is a continuous challenge, particularly given the current importance of RNAs in the control of genome expression. Here, we show that a fluorogenic RNA-DNA hybrid is a powerful tool for a real-time fluorescence detection and assay of exoribonucleases (RT-FeDEx). This RT-FeDEx assay provides a new strategy for the isolation, purification, and assay of known and unknown exoribonucleases.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available
Share Paper
