Journal
RNA BIOLOGY
Volume 11, Issue 12, Pages 1568-1585Publisher
TAYLOR & FRANCIS INC
DOI: 10.4161/15476286.2014.992280
Keywords
biological ribonucleic acids; LC-MS; MS; MALDI-MS; MS; modified RNA; modified bases; RNA sequencing; rRNA; tRNA; tandem mass spectrometry
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Funding
- National Science Foundation [CHE1212625]
- National Institutes of Health [GM58843, RR27671]
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [1212625] Funding Source: National Science Foundation
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The analysis of ribonucleic acids (RNA) by mass spectrometry has been a valuable analytical approach for more than 25years. In fact, mass spectrometry has become a method of choice for the analysis of modified nucleosides from RNA isolated out of biological samples. This review summarizes recent progress that has been made in both nucleoside and oligonucleotide mass spectral analysis. Applications of mass spectrometry in the identification, characterization and quantification of modified nucleosides are discussed. At the oligonucleotide level, advances in modern mass spectrometry approaches combined with the standard RNA modification mapping protocol enable the characterization of RNAs of varying lengths ranging from low molecular weight short interfering RNAs (siRNAs) to the extremely large 23 S rRNAs. New variations and improvements to this protocol are reviewed, including top-down strategies, as these developments now enable qualitative and quantitative measurements of RNA modification patterns in a variety of biological systems.
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