Journal
ONCOTARGET
Volume 6, Issue 26, Pages 21934-21949Publisher
IMPACT JOURNALS LLC
DOI: 10.18632/oncotarget.4290
Keywords
lncRNA; HOTAIR; miR-326; glioma; FGF1
Categories
Funding
- Natural Science Foundation of China [81172197, 81171131, 81272564, 81272795, 81372484, 81372682, 81201800]
- Shenyang Science and Technology Plan Projects [F13-220-9-15, F13-316-1-16, F13-316-1-19]
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Glioma is the most common and aggressive primary adult brain tumor. Long non-coding RNAs (lncRNAs) have important roles in a variety of biological properties of cancers. Here, we elucidated the function and the possible molecular mechanisms of lncRNA HOTAIR in human glioma U87 and U251 cell lines. Quantitative RT-PCR demonstrated that HOTAIR expression was up-regulated in glioma tissues and cell lines. Knockdown of HOTAIR exerted tumor-suppressive function in glioma cells. Further, HOTAIR was confirmed to be the target of miR-326 and miR-326 mediated the tumor-suppressive effects of HOTAIR knockdown on glioma cell lines. Moreover, over-expressed miR-326 reduced the FGF1 expression which played an oncogenic role in glioma by activating PI3K/AKT and MEK 1/2 pathways. In addition, the in vivo studies also supported the above findings. Taken together, knockdown of HOTAIR up-regulated miR-326 expression, and further inducing the decreased expression of FGF1, these results provided a comprehensive analysis of HOTAIR-miR-326-FGF1 axis in human glioma and provided a new potential therapeutic strategy for glioma treatment.
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