Journal
ONCOTARGET
Volume 7, Issue 4, Pages 4746-4759Publisher
IMPACT JOURNALS LLC
DOI: 10.18632/oncotarget.6621
Keywords
glioblastoma multiforme (GBM); non coding RNAs (ncRNAs); microRNAs (miRNAs); circular RNAs (circRNAs); cell networks
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Funding
- MIUR (Italian Ministry for Instruction, University and Research)
- Fondazione Compagnia di San Paolo Torino
- Italian Ministry of Health, Core Grant Ricerca Corrente
- Italian Ministry of Health [GR-2008-1143408]
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MiR-671-5p is encoded by a gene localized at 7q36.1, a region amplified in human glioblastoma multiforme (GBM), the most malignant brain cancer. To investigate whether expression of miR-671-5p were altered in GBM, we analyzed biopsies from a cohort of forty-five GBM patients and from five GBM cell lines. Our data show significant overexpression of miR-671-5p in both biopsies and cell lines. By exploiting specific miRNA mimics and inhibitors, we demonstrated that miR-671-5p overexpression significantly increases migration and to a less extent proliferation rates of GBM cells. Through a combined in silico and in vitro approach, we identified CDR1-AS, CDR1, VSNL1 as downstream miR-671-5p targets in GBM. Expression of these genes significantly decreased both in GBM biopsies and cell lines and negatively correlated with that of miR-671-5p. Based on our data, we propose that the axis miR-671-5p / CDR1-AS / CDR1 / VSNL1 is functionally altered in GBM cells and is involved in the modification of their biopathological profile.
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