Journal
ONCOTARGET
Volume 6, Issue 35, Pages 38139-38150Publisher
IMPACT JOURNALS LLC
DOI: 10.18632/oncotarget.5709
Keywords
miR-124; miR-506; colorectal cancer; DNMT3B; DNMT1
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Funding
- National Natural Science Foundation of China [81302076, 81402249]
- Major Projects of the Natural Science Foundation of Hunan Province [12JJ2054]
- Key Projects of Scientific, Technological Projects in Hunan Province [2013FJ2017]
- State key program of clinical specialty
- Open-End Fund for the Valuable and Precision Instruments of Central South University
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miR-124 and miR-506 are reportedly down-regulated and associated with tumor progression in many cancers, but little is known about their intrinsic regulatory mechanisms in colorectal cancer (CRC). In this study, we found that the miR-124 and miR-506 levels were significantly lower in human CRC tissues than in controls, as indicated by qRT-PCR and in situ hybridization histochemistry. We also found that the overexpression of miR-124 or miR-506 inhibited tumor cell progression and increased sensitivity to chemotherapy in vitro. Increased miR-124 or miR-506 expression also inhibited tumor cell proliferation and invasion in vivo. Luciferase reporter assays and western blotting were used to determine the association between miR-124, miR-506 and their target genes, DNMTs. We further identified that miR-124 and miR-506 directly targeted DNMT3B and indirectly targeted DNMT1. The overexpression of miR-124 and miR-506 reduced global DNA methylation and restored the expression of E-cadherin, MGMT and P16. In conclusion, our data showed that miR-124 and miR-506 inhibit progression and increase sensitivity to chemotherapy by targeting DNMT3B and DNMT1 in CRC. These findings may provide novel avenues for the development of targeted therapies.
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