Journal
ONCOTARGET
Volume 6, Issue 20, Pages 18012-18026Publisher
IMPACT JOURNALS LLC
DOI: 10.18632/oncotarget.4000
Keywords
notch2 receptor; miR-23b; Ets1; E2F1; gastric carcinogenesis
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Funding
- National Science Council [NSC 101-2320-B-010-067-MY3]
- Ministry of Education, Aim for the Top University Plan [103AC-T403, 103AC-T607]
- Center of Excellence for Cancer Research at Taipei Veterans General Hospital [MOHW103-TD-B-111-02]
- Yen Tjing Ling Medical Foundation [CI-103-14]
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Gastric carcinoma is one of the most common malignancies and the third highest cause of global cancer-related death. Notch2 receptor intracellular domain (N2IC), the activated form of Notch2 receptor, enhances gastric carcinogenesis. MicroRNAs (miRNAs) act as either oncogenes or tumor suppressors in tumorigenesis and crosstalk with Notch pathways. Herein, microRNA-23b (miR-23b) was identified as a Notch2 receptor-related miRNA and its role in gastric carcinogenesis was investigated. Levels of miR-23b in stomach adenocarcinoma samples were down-regulated, whereas those of Notch2 receptor, v-ets erythroblastosis virus E26 oncogene homolog 1 (Ets1), and E2F1 transcripts were up-regulated. Results also showed that N2IC down-regulated miR-23b expression in gastric cancer cells through up-regulating E2F1. The miR-23b inhibited gastric tumorigenesis including growth, viability, epithelial-mesenchymal transition, and abilities of colony formation, migration, invasion, and tumorsphere formation. Mechanistically, miR-23b suppressed tumor progression and pluripotency gene expression and affected tumorsphere ultra-structure in gastric cancer cells via targeting Notch2 receptor or Ets1. Furthermore, miR-23b diminished the xenografted tumor growth and lung metastasis of SC-M1 gastric cancer cells through Notch2 pathway. Our results suggest that Notch2 pathway and miR-23b interplay in a reciprocal regulation loop in gastric cancer cells and this axis plays an important role in gastric carcinogenesis.
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