Journal
ONCOTARGET
Volume 6, Issue 4, Pages 1995-2008Publisher
IMPACT JOURNALS LLC
DOI: 10.18632/oncotarget.3038
Keywords
necoalbaconol; necroptosis; RIPK; TNF alpha; NF-kappa B signaling pathway; ROS
Categories
Funding
- National Key Basic Research Program of China [2009CB522300, 2011CB504305]
- State Key Program of National Natural Science Foundation of China [81430064]
- Hunan Provincial Innovation Foundation for Postgraduate [CX2012B082]
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Necroptosis/regulated necrosis is a caspase-independent, but receptor interacting protein kinase (RIPK)-dependent form of cell death. In previous studies, neoalbaconol (NA), a constituent extracted from Albatrellus confluens, was demonstrated to induce necroptosis in some cancer cell lines. The molecular mechanism of NA-induced necroptosis is described in this research study. We determined that NA-induced cell death is partly dependent on tumor necrosis factor a (TNF alpha) feed-forward signaling. More importantly, NA abolished the ubiquitination of RIPK1 by down-regulating E3 ubiquitin ligases, cellular inhibitors of apoptosis protein 1/2 (cIAP1/2) and TNF alpha receptor-associated factors (TRAFs). The suppression of RIPK1 ubiquitination induced the activation of the non-canonical nuclear factor-kappa B (NF-kappa B) pathway and stimulated the transcription of TNF alpha. Moreover, we also found that NA caused RIPK3-mediated reactive oxygen species (ROS) production and contribution to cell death. Taken together, these results suggested that two distinct mechanisms are involved in NA-induced necroptosis and include RIPK1/NF-kappa B-dependent expression of TNF alpha and RIPK3-dependent generation of ROS.
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