Journal
REVISTA BRASILEIRA DE ZOOTECNIA-BRAZILIAN JOURNAL OF ANIMAL SCIENCE
Volume 42, Issue 1, Pages 1-6Publisher
REVISTA BRASILEIRA ZOOTECNIA BRAZILIAN JOURNAL ANIMAL SCI
DOI: 10.1590/S1516-35982013000100001
Keywords
fish; Loricariidae; oocyte; reproduction; semen
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The objective of this study was to evaluate the effects of water volume and water temperature on the sperm motility duration and the number of spermatozoa, and the water volume on the fertilization rates of oocytes of Rhinelepis aspera. Experiments were carried out to evaluate the effect of semen dilutions (1.74x10(-5), 1.74x10(-4), 1.74x10(-3), 1.74x10(-2), 1.74x10(-1) and 1.00 mL of sperm.mL(-1) of water) and water temperature (5, 10, 15, 20, 25, 30, 35, 40, 45, and 50 degrees C) on spermatozoa motility duration. In addition, the effects of insemination dose (7x10(3), 7x10(4), 7x10(5), 7x10(6) and 7x10(7) spermatozoa.oocyte(-1)) and water volume (1.0, 30.0, 60.0, 90.0 and 120.0 mL water.2.0 mL(-1) oocytes) on the artificial fertilization rates of oocytes were evaluated. The longest sperm motility duration were observed for the semen dilution of 1.74x10(-5) mL semen.mL(-1) water and in water at 5 degrees C. The highest fertilization rates were obtained for insemination doses between 7.00x10(3) and 1.23x10(7) spermatozoa. oocyte(-1) and water volume of 28.11 mL water.2.0 mL(-1) oocytes.
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