Journal
REVIEW OF SCIENTIFIC INSTRUMENTS
Volume 83, Issue 5, Pages -Publisher
AMER INST PHYSICS
DOI: 10.1063/1.4719916
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Funding
- Burroughs Wellcome Fund Career Award at the Scientific Interface
- Hellman Family Faculty
- National Science Foundation (NSF) [DMR-1121053]
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We present the design, calibration, and testing of a magnetic tweezers device that employs two pairs of permanent neodymium iron boron magnets surrounded by low-carbon steel focusing tips to apply large forces to soft materials for microrheology experiments. Our design enables the application of forces in the range of 1-1800 pN to similar to 4.5 mu m paramagnetic beads using magnet-bead separations in the range of 0.3-20 mm. This allows the use of standard coverslips and sample geometries. A high speed camera, custom LED-based illumination scheme, and mechanically stabilized measurement platform are employed to enable the measurement of materials with viscoelastic moduli as high as similar to 1 kPa. (C) 2012 American Institute of Physics. [http://dx.doi.org/10.1063/1.4719916]
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