4.5 Article

Single-molecule binding experiments on long time scales

Journal

REVIEW OF SCIENTIFIC INSTRUMENTS
Volume 81, Issue 8, Pages -

Publisher

AMER INST PHYSICS
DOI: 10.1063/1.3473936

Keywords

-

Funding

  1. Howard Hughes Medical Institute Funding Source: Medline

Ask authors/readers for more resources

We describe an approach for performing single-molecule binding experiments on time scales from hours to days, allowing for the observation of slower kinetics than have been previously investigated by single-molecule techniques. Total internal reflection fluorescence microscopy is used to image the binding of labeled ligand to molecules specifically coupled to the surface of an optically transparent flow cell. Long-duration experiments are enabled by ensuring sufficient positional, chemical, thermal, and image stability. Principal components of this experimental stability include illumination timing, solution replacement, and chemical treatment of solution to reduce photodamage and photobleaching; and autofocusing to correct for spatial drift. (C) 2010 American Institute of Physics. [doi:10.1063/1.3473936]

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.5
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available