Journal
REVIEW OF SCIENTIFIC INSTRUMENTS
Volume 79, Issue 10, Pages -Publisher
AMER INST PHYSICS
DOI: 10.1063/1.2999542
Keywords
cellular biophysics; high-speed optical techniques; laser applications in medicine; microorganisms; radiation pressure; surgery
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Funding
- Philippine Council for Advanced Science and Technology Research and Development (PCASTRD)
- Japan Student Services Organization (JASSO)
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We present optical trapping and surgery of living yeast cells using two operational modes of a single laser. We used a focused laser beam operating in continuous-wave mode for noninvasive optical trapping and manipulation of single yeast cell. We verified that such operational mode of the laser does not cause any destructive effect on yeast cell wall. By changing the operation of the laser to femtosecond-pulsed mode, we show that a tightly focused beam dissects the yeast cell walls via nonlinear absorption. Lastly, using the combined technique of optical microsurgery and trapping, we demonstrate intracellular organelle extraction and manipulation from a yeast cell. The technique established here will be useful as an efficient method for both surgery and manipulation of living cells using a single laser beam.
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