Journal
RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY
Volume 185, Issue 3, Pages 615-624Publisher
ELSEVIER
DOI: 10.1016/j.resp.2012.11.005
Keywords
Collagen; Lung mechanics; Stem cells; Asthma; Growth factor
Categories
Funding
- Centres of Excellence Program (PRONEX-FAPERJ)
- Brazilian Council for Scientific and Technological Development (CNPq)
- Rio de Janeiro State Research Foundation (FAPERJ)
- Coordination for the Improvement of Higher Education Personnel (CAPES)
- Sao Paulo State Research Foundation (FAPESP)
- INCT-INOFAR
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We hypothesized that the route of administration would impact the beneficial effects of bone marrow-derived mononuclear cell (BMDMC) therapy on the remodelling process of asthma. C57BL/6 mice were randomly assigned to two main groups. In the OVA group, mice were sensitized and challenged with ovalbumin, while the control group received saline using the same protocol. Twenty-four hours before the first challenge, control and OVA animals were further randomized into three subgroups to receive saline (SAL), BMDMCs intravenously (2 x 10(6)), or BMDMCs intratracheally (2 x 10(6)). The following changes were induced by BMDMC therapy in OVA mice regardless of administration route: reduction in resistive and viscoelastic pressures, static elastance, eosinophil infiltration, collagen fibre content in airways and lung parenchyma; and reduction in the levels of interleukin (IL)-4, IL-13, transforming growth factor-beta and vascular endothelial growth factor. In conclusion, BMDMC modulated inflammatory and remodelling processes regardless of administration route in this experimental model of allergic asthma. (C) 2012 Elsevier B.V. All rights reserved.
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