Journal
RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY
Volume 184, Issue 3, Pages 257-268Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.resp.2012.07.019
Keywords
Ionocyte; Na+/K+-ATPase; Na+/K+/2Cl(-) cotransporter; Cystic fibrosis transmembrane conductance regulator; Na+/Cl- cotransporter; Na+/H+ exchanger
Categories
Funding
- JSPS KAKENHI [22780183]
- Grants-in-Aid for Scientific Research [22780183] Funding Source: KAKEN
Ask authors/readers for more resources
Teleost fishes are able to acclimatize to seawater by secreting excess NaCl by means of specialized ionocytes in the gill epithelium. Antibodies against Na+/K+-ATPase (NKA) have been used since 1996 as a marker for identifying branchial ionocytes. Immunohistochemistry of NKA by itself and in combination with Na+/K+/2Cl(-) cotransporter and CFTR Cl- channel provided convincing evidence that ionocytes are functional during seawater acclimation, and also revealed morphological variations in ionocytes among teleost species. Recent development of antibodies to freshwater- and seawater-specific isoforms of the NKA alpha-subunit has allowed functional distinction of ion absorptive and secretory ionocytes in Atlantic salmon. Cutaneous ionocytes of tilapia embryos serve as a model for branchial ionocytes, allowing identification of 4 types: two involved in ion uptake, one responsible for salt secretion and one with unknown function. Combining molecular genetics, advanced imaging techniques and immunohistochemistry will rapidly advance our understanding of both the unity and diversity of ionocyte function and regulation in fish osmoregulation. (C) 2012 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available