Journal
RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY
Volume 176, Issue 1-2, Pages 39-49Publisher
ELSEVIER
DOI: 10.1016/j.resp.2011.01.005
Keywords
AQP1; AQP5; Lung injury; Tanshinone IIA
Categories
Funding
- Military Key Projects in the 11th Five-year Plan of China [08G-102]
- Shaanxi Province Key Projects [2008104-08]
- National Natural Science Foundation of China [30770925]
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Aquaporins (AQPs), a family of transmembrane water channels, mediate physiological response to changes of fluid volume and osmolarity. It is still unknown what role of AQPs plays in seawater drowning-induced acute lung injury (ALI) and whether pharmacologic modulation of AQPs could alleviate the severity of ALI caused by seawater aspiration. In our study, the results from RT-PCR and Western blotting showed that intratracheal installation of seawater up-regulated the mRNA and protein levels of AQP1 and AQP5 in lung tissues. Furthermore, we found that treatment of tanshinone IIA (TIIA, one of the main active components from Chinese herb Danshen) significantly reduced the elevation of AQP1 and AQP5 expression induced by seawater in rats, A549 cells and primary alveolar type II cells. Treatment of TIIA also improved lung histopathologic changes and blood-gas indices, and reduced lung edema and vascular leakage. These findings demonstrated that AQP1 and AQP5 might play an important role in the development of lung edema and lung injury, and that treatment with TIIA could significantly alleviate seawater exposure-induced ALI, which was probably through the inhibition of AQP1 and AQP5 over-expression in lungs. (C) 2011 Elsevier B.V. All rights reserved.
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