Optimization of balloon-type bubble bioreactor angle and methyl jasmonate concentration to enhance metabolite production in adventitious roots of Pseudostellaria heterophylla

Adventitious roots of Pseudostellaria heterophylla were cultivated in 3-L balloon-type bubble bioreactors (BTBB). The optimum angle of the base of the bioreactor was 90A degrees. The rate of growth (64.96) and electrical conductivity (2.3 mu S cm(-1)) were highest in the culture with the 90A degrees bioreactor angle, and total saponins (9.61 mg g(-1)), polysaccharides (128.29 mg g(-1)) and amino acids (0.25 mg g(-1)) content were also maximum. We also investigated the effects of a single elicitor (methyl jasmonate; MJ) on accumulation of metabolites in P. heterophylla adventitious root culture in 3-L BTBB. The highest rate of growth (21.4) was achieved in the control group, in which the highest amino acids yield was 1.39 mg L-1. The highest total saponins (20.98 mg L-1) and polysaccharides (1.18 mg L-1) yields were obtained after addition of 10 mg L-1 MJ, and were, respectively, 1.28 and 1.23-fold higher than in the control group.