Journal
RESEARCH IN VETERINARY SCIENCE
Volume 97, Issue 2, Pages 182-186Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.rvsc.2014.07.019
Keywords
Salmonella enterica; Efflux pump; Fluoroquinolone; Antimicrobial resistance
Categories
Funding
- Brain Korea 21 (BK21) [T1300291]
- National Antimicrobial Resistance Management Program of the Ministry of Food and Drug Safety [11092NARMP158]
- Rural Development Administration (RDA) [PJ007585]
Ask authors/readers for more resources
Multidrug-resistant foodborne pathogens are a leading public health concern, as antimicrobial resistance can lead to therapeutic failure. In this study, a ciprofloxacin-susceptible Salmonella Istanbul (Sal10-FC-KU12) was isolated from chicken meat obtained from a market in Korea to induce ciprofloxacin-resistant mutants (SalML, SalMM, and SalMH). Minimum inhibitory concentrations (MICs) of 12 antibiotics were measured in the presence or absence of an efflux pump inhibitor. Expression levels of efflux pump-related genes (acrB, acrF, marA, ramA, rob, and soxS) were determined using quantitative reverse transcription polymerase chain reaction (qRT-PCR). Elevated MICs for the derived mutants were shown to result from the action of the efflux pump, with increased expression of marA, ramA, and acrB compared with the wild-type strain. The results of this study suggest that continued use of ciprofloxacin might induce the emergence of Salmonella mutants resistant not only to fluoroquinolones, but also to several other classes of antimicrobials. (C) 2014 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available