Journal
RESEARCH IN VETERINARY SCIENCE
Volume 95, Issue 2, Pages 758-763Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.rvsc.2013.06.001
Keywords
Bacteriophage; Mastitis; Myoviridae; SAH-1; Staphylococcus aureus
Categories
Funding
- Korean Research Foundation Grant [KRF-2008-331-E00385]
- National Research Foundation of Korea [2008-331-E00385] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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A lytic bacteriophage (phage), designated SAH-1, was isolated from sewage effluent near a dairy cow farm in Gwacheon, South Korea to search for biocontrol agents against Staphylococcus aureus infections. The SAH-1 was morphologically classified as Myoviridae and possessed an approximate 144 kb double-stranded genomic DNA. The phage showed broad host ranges within S. aureus strains including methicillin-resistant strains, and its latent period and burst size were approximately 20 min and 100 PFU/cell, respectively. Moreover, morphologic and genomic analysis of SAH-1 revealed that the phage was closely related to other Myoviridae phages infecting Staphylococcus species. The bacteriolytic activity of phage SAH-1 at a multiplicity of infection (MOI) 1 and 100 indicated its efficiency for reducing bacterial growth. Based on these results, phage SAH-1 could be considered a potential therapeutic or prophylactic candidate against S. aureus infections. (C) 2013 Elsevier Ltd. All rights reserved.
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