Journal
RESEARCH IN VETERINARY SCIENCE
Volume 95, Issue 1, Pages 123-129Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.rvsc.2013.03.002
Keywords
Mycobacterium avium subsp paratuberculosis; Paratuberculosis; Johne's disease; Antibody ELISA; PBMC; Lipid antigen
Categories
Funding
- Meat and Livestock Australia
- Cattle Council of Australia
- Sheep Meat Council of Australia
- Wool Producers Australia through Animal Health Australia
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Paratuberculosis caused by Mycobacterium avium subsp. paratuberculosis (MAP) is a chronic infectious disease affecting domestic and wild ruminants. Antigens currently used for the diagnosis of paratuberculosis are whole-cell derived crude preparations. The identification of MAP-specific antigens for the specific and early diagnosis of this infection is strongly needed. This study assessed the ability of the MAP-specific synthetic lipopeptide antigen Para-LP-01 to invoke specific serum antibody (Ab) and cell-mediated immune (CMI) responses in sheep experimentally exposed to MAP S strain. Responses were compared to those elicited by the crude whole-cell derived MAP 316v antigen (316v). Para-LP-01 induced a significant serum Ab response in MAP-infected sheep in comparison with unexposed or uninfected sheep, but failed to induce detectable CMI responses including production of IFN-gamma, IL-10 and lymphoproliferation, unlike 316v which invoked both CMI and serum Ab responses in MAP-exposed sheep. Para-LP-01 is a suitable antigen for serodiagnosis of MAP-infection in sheep. The differential induction of humoral and CMI responses by lipid based antigens could enhance current understanding of the role played by cell-wall associated lipid antigens in the pathogenesis of MAP-infection. (c) 2013 Elsevier Ltd. All rights reserved.
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