Journal
RESEARCH IN VETERINARY SCIENCE
Volume 94, Issue 3, Pages 478-483Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.rvsc.2012.10.002
Keywords
Sepsis; Pathogen associated molecular patterns; Inflammatory mediators
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The N-terminal portion of pro C-type natriuretic peptide (NT-pCNP) has shown promise as a biomarker for sepsis in humans and dogs, however the mechanism of NT-pCNP production in dogs is unknown. Canine aortic endothelial cells were stimulated with lipopolysaccharide, lipoteichoic acid, peptidoglycan, TNF-alpha, IL-beta, IL-6, IL-10, IL-21, CXCL-8, IFN-gamma, VEGF-A or control (PBS), and NT-pCNP production was measured. Lipopolysaccharide, TNF-alpha, and IL-1 beta significantly stimulated NT-pCNP production in a dose and time dependent manner; IL-1 beta resulted in the greatest NT-pCNP concentrations. The other stimulants did not result in significant NT-pCNP production. The addition of serum to the cell culture model did not alter lipopolysaccharide, lipoteichoic acid or peptidoglycan induced NT-pCNP production. These data indicate that lipopolysaccharide, TNF-alpha and IL-1 beta regulate CNP production from canine vascular endothelium and of the stimulants tested, IL-1 beta is the predominant inducing factor. These data provide some initial insight into the mechanisms of CNP regulation in dogs. (C) 2012 Elsevier Ltd. All rights reserved.
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