Journal
RESEARCH IN VETERINARY SCIENCE
Volume 93, Issue 3, Pages 1481-1486Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.rvsc.2012.02.013
Keywords
Mesenchymal stem cell; Scaffold; Gelatin; beta-Tricalcium phosphate; Horse
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A three dimensional scaffold is essential in mesenchymal stem cells (MSCs) delivery in cell-based therapy for facilitating cell adherence, migration, proliferation, and differentiation. The objectives of this study were to evaluate the possibility of beta-tricalcium phosphate incorporated gelatin sponges (Gelatin/beta-TCP sponge) as scaffolds for equine MSCs and to examine the effects of seeding density and seeding method on the proliferation of equine MSCs in the Gelatin/beta-TCP sponges. Mononuclear cells and MSCs isolated from bone marrow were seeded into Gelatin/beta-TCP sponges at different densities by different seeding methods-static or agitated methods. Proliferation of the MSCs in Gelatin/beta-TCP was assessed using the Cell Counting Kit-8 assay and histological examination. Distribution and proliferation of MSCs in the Gelatin/beta-TCP sponge were observed, and the Gelatin/beta-TCP sponge supported limited growth when seeded at high density. We also found that the agitated seeding method enhanced the proliferation of MSCs. This study demonstrated the suitability of Gelatin/beta-TCP sponges for the proliferation and maintenance of equine MSCs. These results contribute to the application of MSC-seeded Gelatin/beta-TCP sponges in equine medicine. (C) 2012 Elsevier Ltd. All rights reserved.
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