Journal
RESEARCH IN VETERINARY SCIENCE
Volume 93, Issue 3, Pages 1190-1194Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.rvsc.2012.06.006
Keywords
Intramuscular fat (IMF); Intramuscular preadipocytes; Gene expression; Pig
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Funding
- Yunnan Natural Science Foundation, China [2009CD056]
- National Natural Science Foundation of China [30660132, 31060331]
- Special Program for Key Basic Research of the Ministry of Science and Technology, China [2007CB116201]
- National Key Program of Transgenic Project of China [2009ZX08009-140B]
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Intramuscular fat (IMF) content plays an important role in meat quality. Triglyceride (TG) metabolism in intramuscular adipocytes is strongly associated with the intramuscular fat deposition. To better understand the mechanisms leading to IMF deposition we compared the expression levels of genes related to preadipocyte differentiation and lipogenesis in the intramuscular preadipocytes isolated from the longissimus muscle of Wujin and Landrace pigs. The results showed that the intramuscular preadipocytes could differentiate into mature adipocytes in vitro. Triglyceride content in adipocytes isolated from Wujin pigs was higher than Landrace pigs during the middle and later phases of preadipocyte differentiation. The expression levels of genes related to preadipocyte differentiation such as PPARG and CEBPA showed differential expression between Wujin and Landrace porcine adipocytes during the early stage of differentiation. The expression levels of lipogenic genes such as FASN and SREBF1 were significantly higher in Wujin porcine intramuscular preadipocytes than in Landrace intramuscular preadipocytes at the middle and the later stages of differentiation. This suggests that preadipocyte differentiation and lipogenesis exhibited breed-related scheduling. (C) 2012 Elsevier Ltd. All rights reserved.
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