Journal
RESEARCH IN VETERINARY SCIENCE
Volume 92, Issue 1, Pages 53-59Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.rvsc.2010.10.008
Keywords
Retention of fetal membranes; Cyp19 gene; Promoter methylation; Estrogen; Cotyledons
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Funding
- Department of Biotechnology (DBT)
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Retention of fetal membranes (RFM) is the major post-partum disorder in dairy cattle. Cyp19 gene encodes the aromatase enzyme responsible for catalyzing the rate limiting step in estrogen biosynthesis, an important hormone for placental maturation and expulsion. The present study was aimed for comparative analysis of Cyp19 gene expression and its epigenetic regulation in placental cotyledons of animals with and without RFM. Significantly lower expression of Cyp19 gene was found in placental samples of RFM affected animals in comparison to normal animals. Methylation analysis of 5 CpG dinucleotides of placenta specific Cyp19 gene promoter 1.1 and proximal promoter, PII showed hypo-methylation of both PI.1 and PII in term placenta of normal and diseased animals. In conclusion, a mechanism other than promoter methylation is responsible for decreased aromatase expression in placental cotyledons of animals suffering from RFM. (C) 2010 Elsevier Ltd. All rights reserved.
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