Journal
RESEARCH IN MICROBIOLOGY
Volume 162, Issue 10, Pages 1027-1032Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.resmic.2011.08.002
Keywords
Landfills; Earthworms; Methanotrophs; pmoA microarray
Categories
Funding
- NERC [NE/B505389/1]
- Society for General Microbiology
- Engineering and Physical Sciences Research Council [EP/F016727/1] Funding Source: researchfish
- Natural Environment Research Council [NE/B505389/1, NE/B505362/1, CEH010021, ceh010023] Funding Source: researchfish
- EPSRC [EP/F016727/1] Funding Source: UKRI
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Landfills represent a major source of methane in the atmosphere. In a previous study, we demonstrated that earthworm activity in landfill cover soil can increase soil methane oxidation capacity. In this study, a simulated landfill cover soil mesocosm (1 m x 0.15 m) was used to observe the influence of earthworms (Eisenia veneta) on the active methanotroph community composition, by analyzing the expression of the pmoA gene, which is responsible for methane oxidation. mRNA-based pmoA microarray analysis revealed that earthworm activity in landfill cover soil stimulated activity of type I methanotrophs (Methylohacter, Methylomonas, Methylosarcina spp.) compared to type II methanotrophs (particularly Methylocystis spp.). These results, along with previous studies of methanotrophs in landfill cover soil, can now be used to plan in situ field studies to integrate earthworm-induced methanotrophy with other landfill management practises in order to maximize soil methane oxidation and reduce methane emissions from landfills. (C) 2011 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
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