Journal
RESEARCH IN MICROBIOLOGY
Volume 159, Issue 6, Pages 458-461Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.resmic.2008.04.011
Keywords
Escherichia coli; acid stress; cyclopropane fatty acid (CFA); survival; H+ flux; pH homeostasis
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Funding
- University of Tasmania [R0013774]
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The physiological role of cyclopropane fatty acids (CFAs) in acid stress resistance was studied by in situ microelectrode H+ flux measurements of Escherichia coli Frag1 and its isogenic CFA-deficient mutant JBM 1. After exposure to pH 4 for 16 h, net H+ influx in JBM1 was twice that of the parent strain. H+ flux stabilisation at pH 6 after acid stress took more time in the cfa(-) mutant. The data suggest increased proton permeability and decreased ability to extrude H+ in the absence of CFA, and they support the hypothesis that CFAs protect E. coli in acidic environments by decreasing membrane permeability to H+. Crown Copyright (C) 2008 Published by Elsevier Masson SAS. All rights reserved.
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