Journal
RESEARCH IN MICROBIOLOGY
Volume 159, Issue 7-8, Pages 562-568Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.resmic.2008.07.003
Keywords
Flagella; Catabolite repression; cAMP; Biofilm; Serratia
Categories
Funding
- NIH [EY08098]
- Eye and Ear Institute of Pittsburgh
- Research to Prevent Blindness
- Campbell Laboratory of Ocular Microbiology
- NATIONAL EYE INSTITUTE [P30EY008098] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R21AI074402] Funding Source: NIH RePORTER
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Serratia marcescens is an emerging opportunistic pathogen with a remarkably broad host range. The cAMP-regulated catabolite repression system of S. marcescens has recently been identified and demonstrated to regulate biofilm formation through the production of surface adhesions. Here we report that mutations in components of the catabolite repression system (cyaA and crp) eliminate flagellum production and swimming motility. Exogenous CAMP was able to restore flagellum production to adenylate cyclase Mutants, as determined by transmission electron microscopy and PAGE analysis. A transposon-generated suppressor Mutation of the crp motility defect mapped to upstream of the fthDC operon. This suppressor Mutation resulted in an upregulation of fthD expression and flagellum production, indicating that fthDC expression is Sufficient to restore flagellum production to crp Mutants. Lastly, and contrary to a previous report, WC found that fthD expression is controlled by the catabolite repression system using quantitative RT-PCR. Together, these data indicate that flagellum production is regulated by the cAMP-dependent catabolite repression system. Given the role of flagella in bacterial pathogenicity. the re regulatory pathway described here may assist us in better understanding the putative role of motility in dissemination and Virulence of this opportunistic pathogen. (C) 2008 Elsevier Masson SAS. All rights reserved.
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