Journal
RESEARCH IN MICROBIOLOGY
Volume 159, Issue 9-10, Pages 579-589Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.resmic.2008.08.007
Keywords
Paracoccus sulfur oxidation pathway; Rhizosphere; soxB; Thiosulfate-oxidizing bacteria; S4 intermediate pathway
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Funding
- Korea Research Foundation
- Brain Korea [BK21]
- National Institute of Agricultural Science and Technology, Rural Development Administration, Republic of Korea
- Rural Development Administration (RDA), Republic of Korea [naas20080330] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Twenty-one thiosulfate-oxidizing bacteria were isolated from rhizosphere soils and 16S rRNA analysis revealed that the isolates were affiliated with seven different phylogenetic groups within the Beta and Gamma subclasses of Proteobacteria and Actinobacteria. Among these, five genera, including Dyella, Burkholderia, Alcaligenes, Microbacterium and Leifsonia sp., represented new sulfur oxidizers in rhizosphere soils. The thiosulfate-oxidizing Dyella, Burkholderia, Alcaligenes, Microbacterium, Leifsonia and Pandoraea were able to grow chemolithotrophically with a medium containing thiosulfate and exhibited growth coupled with thiosulfate oxidation. They accumulated intermediate products such as sulfur, sulfite and trithionate in the spent medium during the time course of thiosulfate oxidation, and these products were finally oxidized into sulfate. Furthermore, they possessed thiosulfate-metabolizing enzymes such as rhodanese, thiosulfate oxidase, sulfite oxidase and trithionate hydrolase, suggesting that these bacteria use the 'S4 intermediate' (S4I) pathway for thiosulfate oxidation. Phylogenetic analysis of the soxB gene revealed that Pandoraea sp. and Pandoraea pnomenusa strains formed a separate lineage within Betaproteobacteria. (C) 2008 Elsevier Masson SAS. All rights reserved.
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