4.3 Article

Protein and DNA fingerprinting of a soil bacterial community inoculated into three different sterile soils

Journal

RESEARCH IN MICROBIOLOGY
Volume 159, Issue 4, Pages 231-236

Publisher

ELSEVIER SCIENCE BV
DOI: 10.1016/j.resmic.2008.03.004

Keywords

soil microbiology; bacterial community; metaproteome; protein fingerprinting; DNA fingerprinting

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The functional and genetic structures of a soil bacterial community were characterized after inoculation into three different sterile soils using a protein and DNA fingerprinting method, respectively. Principal component analysis (PCA) of profiles revealed that, depending on soil characteristics, bacterial communities with similar genetic structures harbored different functional structures and thus could potentially be of differing ecological significance for soil functioning. Co-inertia analysis between protein fingerprinting data and the corresponding sets of soil physicochemical characteristics demonstrated the correlation between the functional structure of the bacterial community and soil parameters, with pH, clay and CaCO3 contents being the most discriminating factors. (C) 2008 Elsevier Masson SAS. All rights reserved.

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